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KMID : 0380020100250030257
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Korean Journal of Biotechnology and Bioengineering
2010 Volume.25 No. 3 p.257 ~ p.264
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Expression and Purification of Recombinant Human Bone Morphogenetic Protein-7 (rhBMP-7) in Bacillus subtilis
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Kim Chun-Kwang
Oh Sung-Duk
Lee Jong-Il
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Abstract
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Bone morphogenetic protein-7 (BMP-7) is one of important growth factors for skeletal development and bone growth. In this work, BMP-7 was efficiently expressed in recombinant Bacillus subtilis. The mature BMP-7 protein indicated molecular weight of 15.4 kDa by Western blot assay and was secreted into culture medium with 0.35 ng/mL. The extracellular and intracellular rhBMP-7 proteins were purified by using a FPLC system with an ion exchange column and a gel filtration column. The extracellular and intracellular rhBMP-7 proteins had finally a 57.1% purity and a 36.2% purity, respectively. The purified rhBMP-7 proteins showed an intact biological activity which stimulated alkaline phophatase (ALP) activity in MC3T3-E1 cells.
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KEYWORD
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Bacillus subtilis, expression, bone morphogenetic protein-7, purification
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